On-card Dry Reagent Storage for Disposable Microfluidic Immunoassays
نویسندگان
چکیده
Point-of-care microfluidic assays for the developing world face uncontrolled storage temperatures that can destroy functionality of on-card reagents. In development of such assays [1], we report a method for dry storage and microfluidic rehydration of immunochemical reagents in a sugar-based matrix on porous media. Using gold-antibody conjugates against the malarial antigen Plasmodium falciparum histidine-rich protein II, we identify preservatives for long-term reagent viability on porous substrates, demonstrate repeatable reagent rehydration in microfluidic formats, and characterize microfluidic reagent release.
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